Download frozen tubifex worms8/26/2023 ![]() However, some may exhibit a prolonged lag period and should be given twice the normal incubation time before discarding as nonviable. Given proper treatment and conditions, most freeze-dried cultures will grow out in a few days. Incubate cultures under the appropriate conditions. Please support our sponsors and let them know you heard about them on. Please note that anaerobic bacterial cultures must be rehydrated in an anaerobic environment the viability of the cells decrease rapidly if the vial is rehydrated in an oxygenic environment. Alternatively, algal cultures must be initiated on agar plates. The last few drops of this suspension may also be transferred to an agar slant. Use this to rehydrate the entire pellet, and transfer the entire suspension back into the broth tube and mix well. For freeze dried cultures, using a single tube of the recommended media (5 to 6 mL), withdraw approximately 0.5 to 1.0 mL with a Pasteur or 1.0 mL pipette. The preferred method for long-term preservation of bacteria and algae is freeze-drying however, some bacteria do not survive freeze-drying well and are frozen instead. In microbiology you revive freeze-fried bacteria. During peak summer months (July and August), where the average temperatures around the country are above 25C, we may withhold delivery of live and frozen foods. Please note: standard weight postage for this item covers up to 6 bags of mixed live food. Some companies, like Hikari, sterilize before freeze drying. A bag of tubifex worms typically contains around 200 worms (usually together in a ball).
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